Cspa induction temperature
WebThe 5′ end of the cspA mRNA contains a regulatory sequence (cold box), which stabilizes the mRNA at low temperature, enabling cold shock induction (Xia et al., 2002). The … WebJun 11, 2024 · induction of CspA at low temperature (Phadtare and Severinov, 2005). The 5'-UTR was shown to be responsible for the extreme instability of cspA mRNA at 37°C (a half-life of 12 s) and has a positive effect on its stabilization upon cold shock (a half-life of more than 20 min) (Mitta et al., 1997). The
Cspa induction temperature
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WebOverexpression of proteins in Escherichia coli at low temperature improves their solubility and stability. Here, we apply the unique features of the cspA gene to develop a series of … WebThe expression of cspB, unlike the other homologues, is cold‐shock inducible like cspA. Results: We examined the cold‐shock induction of CspA and CspB at various temperatures. The cspA induction is observed by temperature shift from 37 to 30 °C and high levels of CspA production are observed between 24 and 10 °C. In contrast, CspB ...
WebJan 15, 2010 · Cold induction of cspA, the paradigm Escherichia coli cold-shock gene, is mainly subject to posttranscriptional control, partly promoted by cis-acting elements of its … WebMar 1, 2002 · Among them, CspA is the major cold shock protein because of its highest induction (more than 10% of the total cellular protein synthesis) after temperature …
WebJan 15, 2010 · Cold induction of cspA, the paradigm Escherichia coli cold-shock gene, is mainly subject to posttranscriptional control, partly promoted by cis-acting elements of its transcript, whose secondary structure at … WebA transcriptional gene fusion between the cspA promoter and the lacZ gene was constructed to assess the usefulness of cold shock promoters for low-temperature …
WebMar 15, 1999 · As with cspA mRNA, the cold induction of CspA also depended on the initial level of this protein in cells at different stages of growth and the maximum increment (30-fold) was seen in mid-to-late log cells . In all cases, however, the maximum level attained by CspA after cold shock (≈2% of total soluble protein) is such that the net …
WebDec 1, 1999 · CspA induction occurs over the broadest temperature range (30 ° C to 10 ° C), CspI induction occurs over the narrowest and lowest range (15 ° C to 10 ° C), and florida department of transportation outdoorWebAug 19, 2015 · The cspA-derived 5′UTR region allows low-temperature induction as it is stable at temperatures around 15 °C, but highly unstable at 37 °C. A TEV protease recognition sequence was introduced at the Bam HI restriction site of the cloning region of pCold-II to allow removal of the vector-encoded His-tag from recombinant proteins. great wall autoscoutWebJan 23, 2007 · Cold shock protein CspA. Gene. cspA. Status. UniProtKB reviewed (Swiss-Prot) Organism. Escherichia coli (strain K12) ... Induction. In response to low temperature (at protein level) (PubMed:2404279, PubMed:8898389). 2 publications. Interaction. florida department of transportation pay tollWebApr 1, 1999 · CspI induction at lower temperatures. CspA, CspB, CspG, and CspI are all cold shock inducible. However, upon cold. shock, the temperature dependence of CspA induction is broad- florida department of transportation detailsWebNov 1, 1997 · The regulation of the cold shock induction of CspA and its homologs occurs at the levels of transcription, mRNA stability, and translation. ... We show that at low … florida department of unemployment taxWebFollowing temperature downshift, CspA, CspB, CspG and CspI are highly induced, as indicated by the arrows in Fig. 2C (Wang et al. 1999). However, interestingly, the … florida department of transportation logoWebJan 15, 2010 · cspA and cspD mRNAs were analyzed by temperature gradient gel electrophoresis: (C) electrophoretic mobility in a 7.5% acrylamide gel of cspA mRNA (428 nt) and cspD mRNA (372 nt) as a … florida department of transportation safety