Cy5 glen research

Author: ojdf

August undefined, 2024

WebThe deprotection step is carried out at 65°C for 5 minutes. Deprotection can also be carried out at lower temperatures as shown in Table 2. In all cases, no base modification has been observed. Figure 1 illustrates the differences in RP HPLC between partially and fully deprotected oligos, DMT-off and DMT-on. Table 2. WebHowever, for dyes emitting in the far red, such as Cy5, the BHQ dyes were far superior – quenching the Cy5 with 96% efficiency, compared to 84% with Dabcyl. This may reflect the BHQ's ability to form stable, non-fluorescent complexes which can be a plus even in FRET probes. Indeed, recent work suggests that these non-fluorescent complexes ...

dA-CE Phosphoramidite - Glen Research

WebCy5-labeled aza-peptidyl Pro-Asn epoxide. Cy5-Regioselectively addressable functionalized template- [cyclo- (RGD- d -Phe-Lys)] 4 peptide. Cy5-Tat-Glu-Pro-Asp-acyloxymethyl ketone. L36-Cy5 anti-laminin … Webstable during deprotection, is widely used in the research.10 Here we report the study of Cy5/BHQ2, Cy5/BHQ3, sCy5/ ... and BHQ3-CPG were from Glen Research. Oligonucleotides ft. bliss id card office

Cy5 5 Glen Research Bioz

WebGlen Research Technical Bulletin Extinctions3.html[7/16/19, 11:32:03 AM] EXTINCTION COEFFICIENTS AND FLUORESCENCE DATA Calculate extinction coefficient of an oligo by either summing up the extinction coefficients of the individual bases times their number of occurrences. Or use a formula that takes into account nearest neighbor effects. WebAug 7, 2016 · We performed the comparison of dyes and quenchers (Cy5 vs. disulfo-Cy5 and BHQ2 vs. BHQ3) in several TaqMan probes with different stability of the secondary structure. Based on the studies of ... WebDescription. Glen Research CE (B-cyanoethyl) Phosphoramidites are produced and packaged to ensure the highest performance on DNA synthesizers. Every Glen Research product is accompanied by a Certificate of Analysis and HPLC trace, showing the results of our QC testing. Every Glen Research monomer vial is specially cleaned to eliminate ... ft bliss jag office

Oligonucleotide Synthesis Supplies & Supports Glen …

EXTINCTION COEFFICIENTS AND FLUORESCENCE DATA

WebCy5 dye. Invitrogen Cy5 dye is a bright, far-red-fluorescent dye with excitation ideally suited for the 633 nm or 647 nm laser lines. A significant advantage to using long wavelength dyes such as Cy5 dye or Invitrogen Alexa Fluor 647 dye over other fluorophores is the low autofluorescence of biological specimens in this region of the spectrum. ft bliss inspector generalWebCyanine 3 and Cyanine 5 supports are also offered to allow simpler production of 3’ cyanine dye-labelled oligonucleotides. Deprotection of oligos containing Cyanine dyes … ft bliss in new mexico

"WebDescription. Glen Research CE (B-cyanoethyl) Phosphoramidites are produced and packaged to ensure the highest performance on DNA synthesizers. Every Glen Research product is accompanied by a Certificate of Analysis and HPLC trace, showing the results of our QC testing. Every Glen Research monomer vial is specially cleaned to eliminate ... " - Cy5 glen research

Cy5 glen research

Cy5/BHQ dye–quencher pairs in fluorogenic qPCR probes: …

WebTwo cyanine derivatives, Cyanine 3 and Cyanine 5, which differ in structure simply by the number of carbons in the conjugated poly- ene linkage, are joined by the closely related analogues, Cyanine 3.5 and Cyanine 5.5, and are available as phosphoramidites. Cyanine dyes are normally added once at the 5’-terminus and the MMT group should be ... WebAll solvents and reagents are prepared to our exacting specifications to ensure the highest synthesis efficiency and are passed through a 0.2 micron filter during packaging to eliminate particulate contamination. Glen Research uses freshly sublimed 1H-tetrazole for premium performance on oligonucleotide synthesizers. View Quality Assurance.

Did you know?

WebNov 10, 2007 · Cy5.5 is a NIR fluorescent dye with an absorbance maximum at 675 nm and an emission maximum at 694 nm with a high extinction coefficient of 250,000 M-1 cm-1. … WebGlen Research offers the highest level of Quality Assurance for reagents for DNA and RNA synthesis - Sterling Quality and Performance. We now apply the Sterling criteria of quality and performance to all of Glen Research’s established products. common monomers and supports are available for the variety of synthesizers. Glen Research has developed and …

WebSpectral Characteristics of Fluorescent Dyes. Absorbance Maximum: Emission Maximum: Color: Fluorescein: 494nm: 525nm: Green: Tetrachloro-Fluorescein WebClick here to view our reports! Sign up to receive technical advice and exclusive deals directly to your inbox.

WebCyanine 3 and Cyanine 5 supports are also offered to allow simpler production of 3’ cyanine dye-labeled oligonucleotides. Deprotection of oligos containing Cyanine dyes may be carried out with ammonium hydroxide at room temperature, regardless of the base protecting groups on the monomers used. If there is a need to use ammonium hydroxide … WebGlen Research cyanine 5 cy5 phosphoramidite Cyanine 5 Cy5 Phosphoramidite, supplied by Glen Research, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more

WebGlen Report 34-11: Beyond the UV Region — DEACM-dG as a Versatile Tool for Light-Activatable (“Caged”) Oligonucleotides. Almost all major processes of life on the molecular and cellular scale are inherently three-dimensionally structured and time-dependent. Investigating these aspects requires tools that allow asking spatiotemporal questions.

WebGlen Research cyanine 5 cy5 phosphoramidite Cyanine 5 Cy5 Phosphoramidite, supplied by Glen Research, used in various techniques. Bioz Stars score: 86/100, based on 1 … ft bliss itt tickets officeWeb13 rows · Minimal residual disease research; Drug discovery; Antibody Discovery; Drug … gigachad frogWebCoupling: For labeling 0.2 µmoles of an amino-modified oligonucleotide, dissolve 3.2 mg of the ROX NHS Ester in 100 µL DMSO (or DMF). Dissolve the amino-modified oligonucleotide in 500 µL 0.1 M carbonate/bicarbonate buffer (pH 9). Add 20 µL of the NHS Ester solution and vortex. Let react for 60 min at room temperature and desalt ... gigachad full pictureWebModification and Labeling. Glen Research products for modification and labeling are produced and packaged to ensure the highest performance on commercial synthesizers. Every batch is accompanied by an Analytical Report and an HPLC trace, showing the results of our QC testing. Glen Research monomers are packaged in industry standard vials … ft bliss lemon lotWebEXTINCTION COEFFICIENTS AND FLUORESCENCE DATA Calculate extinction coefﬁcient of an oligo by either summing up the extinction coefﬁcients of the individual bases times their number of occurrences. ft bliss informationWebGlen Research offers a wide selection of fluorophores and fluorescence quenchers, which can be added during oligonucleotide synthesis at the 5′- or 3’-terminus or within the … ft bliss legal servicesWebCy5.5-Conjugated anti-epidermal growth factor receptor monoclonal antibody C225. Cy5.5-Conjugated anti-human CD31 monoclonal antibody. Cy5.5-Conjugated glycol chitosan-5β-cholanic acid nanoparticles. Cy5.5 … ft bliss legal assistance