Fk2 antibody
WebFeb 11, 2024 · Feb 11, 2024 in JST） Size 100 µg/100 µL Citations Data Sheet SDS Clone FK2 has been reported to recognize K29-, K48-, K63-linked poly ubiquitinylated and mono ubiquitinylated proteins but not free ubiquitin (PMID:18757370, 19237541) . The availability is based on the information in Japan at 00:05, Feb 11, 2024 in JST. WebImmunocytochemistry/Immunofluorescence analysis using Mouse Anti-Ubiquitin Monoclonal Antibody, Clone FK2 (SMC-214). Tissue: Fibroblast cell line (NIH 3T3). Species: Mouse. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-Ubiquitin Monoclonal Antibody (SMC-214) at 1:100 for 60 min at RT.
Fk2 antibody
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WebApr 14, 2024 · Ubiquitinated proteins, detected by the monoclonal antibody FK2 (ub-FK2), and RNF8-dependent ubiquitinated H2A (ub-H2A, detected by monoclonal antibody E6C5) are localized on the sex body area (Lu et al., 2010). K48- and K63-linked poly-ubiquitins are enriched in the XY body (Lu et al., 2010). The role of ubiquitination around the XY body ...
WebJan 7, 2024 · product name : Mono- and polyubiquitinylated conjugates monoclonal antibody (FK2) catalog : BML-PW8810-0100 quantity : 100 µg clonality : monoclonal host : mouse conjugate : nonconjugated clone name : FK2 reactivity : southern flounder, Saccharomycetales, human, mouse, rat, fruit fly application : WebNational Center for Biotechnology Information
WebClone FK2 Antibody Products. Clone FK2 is reported to be a monoclonal antibody specific to the human gene, UBB. Indicated applications for antibody clone FK2 include Western … WebSep 15, 2013 · The FK2 antibody is a mouse monoclonal recognizing both monoubiquitinated and polyubiquitinated conjugates. According to the manufacturer’s data sheet the FK2 antibody can recognize various types of ubiquitin chains; however, it is unknown if it has equal affinities for all seven ubiquitin chain linkages and for different …
WebDownload Zoom This Anti-Ubiquitin Mouse mAb (FK2) is validated for use in ELISA, Immunoblotting, Immunoprecipitation, Immunofluorescence for the detection of Ubiquitin. Anti-Ubiquitin Mouse mAb (FK2) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents. SDS CoA References Data Sheet …
WebJun 30, 2024 · For the analysis of human cells, OtUBD pulldowns performed better than FK2 antibody immunoprecipitations in our hands, both in terms of overall efficiency and repeatability. Inconsistency in the performance of antibodies from different batches has been a widely recognized issue . Recombinant OtUBD, by contrast, appeared to be … binary trigger legality by stateWebThis Anti-Ubiquitin Mouse mAb (FK2) is validated for use in ELISA, Immunoblotting, Immunoprecipitation, Immunofluorescence for the … binary triggers for glockWebWestern blots were developed using PVDF membranes and A-106 anti-ubiquitin primary antibody at 0.5 µg/ml followed by HRP-labeled anti-mouse (R&D Systems # HAF007) … binary triggers in ctWebUbiquitin Antibody (FK2) [Unconjugated] Summary. This antibody will recover mono- and polyubiquitinated proteins in I.P. applications. This antibody detects mono- and … binary triggers in floridaWebAnti-Ubiquitinylated proteins Antibody, clone FK1 04-262 Sigma-Aldrich Anti-Ubiquitinylated proteins Antibody, clone FK1 Download Zoom Anti-Ubiquitinylated proteins Antibody, clone FK1 is an antibody against … cyrano movie running timeWeb(B and C) Mono- and poly-ubiquitinated proteins detection using the FK2 specific monoclonal antibody (B) Quantification of ubiquitinated protein detection after normalization to Actin (C). (D) Control and treated RHEs were stained with haematoxylin–eosin (n = 2, 1 independent bank). Bar = 10 μm. cyrano old movieWeb(C) Ubiquitin IP with the FK2 antibody from the cells expressing the indicated proteins, followed by IB with FK2 and the pan-ubiquitin antibody #3933. (D) IP using the HA antibody from the cells co-expressing HA-ubiquitin with the indicated proteins, followed by IB with the HA and pan-ubiquitin antibodies. binary triggers for glocks