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Myh6 cre mouse

WebGenotyping primers for these two new alleles are listed in table S5. Generation and genotyping of the Nkx2.5 cre and Myh6 mercremer lines has been described previously (9, 35, 36). Mice were kept on a mixed C57BL/6:129SVJ background. The experimental MI procedure is described in Supplementary Materials and Methods. Web5 mrt. 2024 · Myh6-MerCreMer:LARP7 f/f mice were injected with 25 mg/kg/d tamoxifen (iCKO) for continuous 5 days at 2 months of age to deplete LARP7 from cardiomyocytes. Two-month-old MerCreMer mice with the same tamoxifen injection plan were used as controls (n=6 to 7; 2-tailed Student t test; ** P <0.01).

Attenuation of Cardiomyocyte Hypertrophy via Depletion

WebGFAP-Cre transgenic mice from founder line 73.12 have Cre recombinase expression directed by the mouse glial fibrillary acidic protein promoter. Cre expression is observed … Web27 jul. 2024 · To genetically label CMs in mice, Rosa26-tdTomato reporter mice ( R26RTdT) 10 were crossed with Myh6-Cre transgenic mice ( Myh6cre; a generous gift from Jeffery Molkentin, Cincinnati Children’s Hospital Medical Center). All mice were maintained on a C57Bl/6J background. 2.1.1 Transverse aortic banding surgery chip shop dunston https://nhukltd.com

Figure 1 from Increased Reactive Oxygen Species–Mediated Ca2 ...

WebTransgenic Mice B6 Fvb 129 A1cftg Myh6 Cre Esr1 1jmk J, supplied by The Jackson Laboratory, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more Average 86 stars, based on 1 article reviews Price from $9.99 to $1999.99 Web15 sep. 2024 · Inducible Cre recombinase using Myh6 promoter has also been established [17, 18]. However, adverse effect of tamoxifen treatment on normal heart function in … Web20 sep. 2024 · The Myh6-Cre mouse line was generated by the Shanghai Model Organisms Center, INC. This newly generated mouse line is available from the … chip shop dumbarton

Evidence for hormonal control of heart regenerative capacity …

Category:009074 - Strain Details - The Jackson Laboratory

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Myh6 cre mouse

GSDMD-Mediated Cardiomyocyte Pyroptosis Promotes …

Web条件性敲除小鼠定义：条件性基因敲除小鼠（也叫Flox小鼠）是指在目的基因中含有成对的loxp位点的小鼠，与Cre工具小鼠交配后可在特定的组织或细胞中敲除目的基因。lCKO如何实现？重组酶系统（如：Cre-loxP）介导的位点特异性重组技术。Cre是重组酶（38kDa），可识别34bp长的DNA序列loxP。 http://lsi.zju.edu.cn/2024/0209/c45388a1959204/page.htm

Myh6 cre mouse

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WebTo test this hypothesis, cardiomyocyte-specific TLR4 knockdown (cTLR4KO) mice were generated by crossing floxed TLR4 mice with Myh6-Cre mice, and subjected to angiotensin II (Ang II, 1 µg/kg/min or vehicle for 14 days) hypertension model. Web靶基因信息：Cre recombinase，Cre (Cyclization Recombination Enzyme,即环化重组酶）是来源于噬菌体P1的一种酶蛋白，分子量约38kDa。. 它可以识别催化两个LoxP位点之间发生同源重组，从而造成DNA的缺失、易位等现象。. 构建信息：该转基因在小鼠心脏特异性α-肌球 …

Web21 mrt. 2024 · Myh6-driven Cre recombinase activates the DNA damage response and the cell cycle in the myocardium in the absence of loxP sites. A New Mouse Model of … WebMyh6 Kcnj8 Tg(Myh6-Kcnj8)634Kcmm GH: Transgene: Diabetes and Obesity Research Cardiovascular Research ... Cre/loxP system: RBRC10016. B6.129S2-Rhoj/76Maim: Rhoj neo: Targeted Mutation: RBRC10015. B6.Cg-Bcl6 loxP Bcl6 EYFP: ... Mouse Models for Human Disease

Web• mutants treated with tamoxifen at 8-9 weeks of age to induce Cre-mediated recombination develop dilated cardiomyopathy decreased cardiac muscle contractility ( J:127391 ) • systolic function of mutants treated with tamoxifen exhibits a 50% or greater decrease in peak aortic flow velocity and both mean and peak acceleration WebIn this study, we generated a Myh6-Cre knockin mouse model by inserting the IRES-Cre-wpre-polyA cassette between the translational stop codon and the 3' untranslated region …

WebHere we present evidence that these αMyHC-Cre (+/-) mice show molecular signs of cardiac toxicity by 3months of age and exhibit decreased cardiac function by 6months of …

http://www.informatics.jax.org/allele/MGI:3779100 graphattentionlayer nn.module :Web21 mei 2024 · Adult cardiomyocytes from the ventricles of Myh6-Cre GSDMD +/+ mice (controls) or GSDMD-CKO (cardiomyocyte-specific GSDMD-deficient) male mice were isolated, and ( A) H/R for 0.5/24 h induced the pyroptotic morphological manifestations of prominent bubble-like formations, as well as propidium iodide (PI)-positive staining of … graph attention networks iclr 2018引用Web27 jan. 2024 · Numerous pharmacotherapies, implantable devices, and surgical techniques have been pioneered over the last five decades to salvage the myocardium and prevent pathological remodeling, dramatically improving patient outcomes and quality of life following the development of HF ( Benjamin et al., 2024; Jones et al., 2024 ). graph attributionchip shop dutiesWeb011038 B6.FVB-Tg(Myh6-cre)2182Mds/J The cardiac-specific alpha myosin-heavy chain ( Myh6 ) promoter drives expression of cre in this transgenic strain. The promoter induces … chip shop dyke roadWeb7 jul. 2024 · Adult cardiomyocytes from the ventricles of Myh6-Cre GSDMD +/+ mice (controls) or GSDMD-CKO (cardiomyocyte-specific GSDMD-deficient) male mice were … graph at which the tangent line is horizontalWebTo examine the role of aggregates in cardiomyopathy, mice with a perinatal onset cardiac selective (Myh6-Cre driven) deletion of an adaptor protein, p62, (p62cKO) were generated. graph auditlog.read.all