WebNCT-503 is a small molecule 3-phosphoglycerate dehydrogenase (PHGDH) inhibitor (IC50=2.5 uM) that reduces the production of glucose-derived serine in cells and suppress the growth of PHGDH-dependent cancer cells.NCT-503 exhibits EC50 of 8-16 uM for the PHGDH-dependent cell lines; has reasonable aqueous solubility and favorable ADME … Web29. apr 2015 · To enable studies of PHGDH function, we report a method to purify recombinant PHGDH and found that untagged enzyme activity was greater than N-terminally tagged enzyme. Analysis of tagged and untagged PHGDH using size exclusion chromatography and electron microscopy found that an N-terminal epitope tag alters …

Discovery of Novel Allosteric Effectors Based on the Predicted ...

WebIt has been reported that R236 is located in the catalytic center of PHGDH and is responsible for PHGDH activity, likely due to its positive charge property that anchors the carboxyl … Web18. jan 2024 · The experiments confirmed that 2 binds to PHGDH with a K d of 35.02 µM, in the same range as the experimentally determined IC 50 value. In contrast, the meta … shelves installation before and after

PHGDH as a mechanism for resistance in metabolically …

WebPhosphoglycerate Dehydrogenase (PHGDH) Activity Assay Kit (Colorimetric) (ab273328) provides a quick and easy method for monitoring PHGDH activity in a variety of samples. … WebThere is an extensive body of literature showing that increased PHGDH activity is important for cancer cell proliferation [32] [33][34] . Accordingly, average high PHGDH gene expression has been ... WebHowever, how the oligomeric states affect PHGDH enzyme activity remains elusive. We studied the dependence of PHGDH enzymatic activity on its oligomeric states. We found … sportswear for women fitness